Journal: Innate Immunity

Article Title: Susceptibility of primary human airway epithelial cells to Bordetella pertussis adenylate cyclase toxin in two- and three-dimensional culture conditions

doi: 10.1177/1753425920979354

Figure Lengend Snippet: Airway epithelial cells do not express CD11b. Expression of CD11b in airway epithelial cells and THP-1 was analysed by flow cytometry. CD11b-positive cells were not found in HNEC (a), HTEC (b) or HBEC3-KT (c) samples. THP-1 populations were CD11b-positive (d). Blue histograms show isotype control-stained cells and red histograms show cells stained with an anti-CD11b Ab. The figure shows the histograms of one representative experiment ( n =3).

Article Snippet: 14, Airway epithelial cells were grown in Airway Epithelial Cell Growth Medium (AECG; #PB-C-MH-350-0099, PeloBiotech, Martinsried, Germany).

Techniques: Expressing, Flow Cytometry, Control, Staining

Journal: Innate Immunity

Article Title: Susceptibility of primary human airway epithelial cells to Bordetella pertussis adenylate cyclase toxin in two- and three-dimensional culture conditions

doi: 10.1177/1753425920979354

Figure Lengend Snippet: CyaA affects intracellular cAMP concentration and IL-6 secretion in human airway epithelial cells. (a) HNEC, HTEC and HBEC3-KT were incubated with CyaA or CyaA-AC – and intracellular cAMP concentration was analysed ( n = 4). (b) Quantification of human IL-6 secreted by HNEC, HTEC and HBEC3-KT populations after treatment with CyaA or CyaA-AC – was assayed by ELISA of the supernatants ( n = 3). (c) Quantification of human IL-6 secreted by human nasal mucosa tissue models after treatment with CyaA or CyaA-AC – ( n =3). All data are presented as means (bars) + SEM (error bars). Differences after CyaA incubation compared to the control group (TUC) are indicated (* P ≤ 0.05, $ P > 0.05 ≤ 0.1, ANOVA). Statistically significant differences of IL-6 basal levels are indicated ( # P < 0.05, ANOVA). Significant cell type-specific differences are indicated ( & P < 0.05, ANOVA).

Article Snippet: 14, Airway epithelial cells were grown in Airway Epithelial Cell Growth Medium (AECG; #PB-C-MH-350-0099, PeloBiotech, Martinsried, Germany).

Techniques: Concentration Assay, Incubation, Enzyme-linked Immunosorbent Assay, Control